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            <itunes:name>eXoZymes</itunes:name>
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            <title>Interview: Cayman Chemical validates eXoZymes' technology and scalability</title>
            <link>http://exozymes.twentythree.com/interview-cayman-chemical-validates</link>
            <description>&lt;p&gt;&lt;p&gt;Using eXoZymes' tech transfer package, Cayman Chemical advanced the protocol from a one-liter setup to a 100-liter pilot run, operating the reaction, downstream processing, and analysis independently. During the run, the team encountered real-world conditions - including pH variation and precipitation - which often cause enzyme-based processes to fail. Despite this, the reaction continued to perform reliably and delivered strong results, ultimately producing more than 500 grams of pharma-grade N-&lt;em&gt;trans&lt;/em&gt;-caffeoyltyramine (NCT) at 99.6% purity, a result that is difficult to obtain in biomanufacturing without extensive purification steps. Additionally, the 99% conversion of feedstock to end product highlights a level of efficiency that is challenging to achieve and maintain in traditional biomanufacturing.&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;For Cayman Chemical, the outcome stood out based on prior experience as highlighted by Patrick Westcott, Director of Catalog Chemistry Production at Cayman Chemical: “&lt;em&gt;I’ve performed a lot of cell-based enzymatic biocatalysis over the years; it is a critical component of what we do at Cayman. One common aspect of this work is a drop in efficacy during scale-up. Anytime you move up in scale orders of magnitude, a process changes enough that it is considered a different process altogether.”&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;Westcott, continues, &lt;em&gt;“During the experiment, from the first sample that we took the analysis showed over a 99% conversion rate. There was some concern because I thought that that maybe was a little bit too high. So, we decided to take a second sample to see if it maintained a similar conversion rate. And we were a little surprised to find out that we actually did achieve this 99% conversion in our first 100-fold scale up of their process. That was very much an aha moment for us!&lt;/em&gt;”&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/interview-cayman-chemical-validates"&gt;&lt;img src="http://exozymes.twentythree.com/64968575/122969142/d406da6a433f2b61ac7a70d1464afefe/standard/download-39-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</description>
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            <pubDate>Wed, 18 Mar 2026 13:59:35 GMT</pubDate>
            <media:title>Interview: Cayman Chemical validates eXoZymes' technology and scalability</media:title>
            <itunes:summary>Using eXoZymes' tech transfer package, Cayman Chemical advanced the protocol from a one-liter setup to a 100-liter pilot run, operating the reaction, downstream processing, and analysis independently. During the run, the team encountered real-world conditions - including pH variation and precipitation - which often cause enzyme-based processes to fail. Despite this, the reaction continued to perform reliably and delivered strong results, ultimately producing more than 500 grams of pharma-grade N-trans-caffeoyltyramine (NCT) at 99.6% purity, a result that is difficult to obtain in biomanufacturing without extensive purification steps. Additionally, the 99% conversion of feedstock to end product highlights a level of efficiency that is challenging to achieve and maintain in traditional biomanufacturing.For Cayman Chemical, the outcome stood out based on prior experience as highlighted by Patrick Westcott, Director of Catalog Chemistry Production at Cayman Chemical: “I’ve performed a lot of cell-based enzymatic biocatalysis over the years; it is a critical component of what we do at Cayman. One common aspect of this work is a drop in efficacy during scale-up. Anytime you move up in scale orders of magnitude, a process changes enough that it is considered a different process altogether.”Westcott, continues, “During the experiment, from the first sample that we took the analysis showed over a 99% conversion rate. There was some concern because I thought that that maybe was a little bit too high. So, we decided to take a second sample to see if it maintained a similar conversion rate. And we were a little surprised to find out that we actually did achieve this 99% conversion in our first 100-fold scale up of their process. That was very much an aha moment for us!”</itunes:summary>
            <itunes:subtitle>Using eXoZymes' tech transfer package, Cayman Chemical advanced the protocol from a one-liter setup to a 100-liter pilot run, operating the reaction, downstream processing, and analysis independently. During the run, the team encountered...</itunes:subtitle>
            <itunes:author>eXoZymes</itunes:author>
            <itunes:duration>08:01</itunes:duration>
            <media:description type="html">&lt;p&gt;&lt;p&gt;Using eXoZymes' tech transfer package, Cayman Chemical advanced the protocol from a one-liter setup to a 100-liter pilot run, operating the reaction, downstream processing, and analysis independently. During the run, the team encountered real-world conditions - including pH variation and precipitation - which often cause enzyme-based processes to fail. Despite this, the reaction continued to perform reliably and delivered strong results, ultimately producing more than 500 grams of pharma-grade N-&lt;em&gt;trans&lt;/em&gt;-caffeoyltyramine (NCT) at 99.6% purity, a result that is difficult to obtain in biomanufacturing without extensive purification steps. Additionally, the 99% conversion of feedstock to end product highlights a level of efficiency that is challenging to achieve and maintain in traditional biomanufacturing.&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;For Cayman Chemical, the outcome stood out based on prior experience as highlighted by Patrick Westcott, Director of Catalog Chemistry Production at Cayman Chemical: “&lt;em&gt;I’ve performed a lot of cell-based enzymatic biocatalysis over the years; it is a critical component of what we do at Cayman. One common aspect of this work is a drop in efficacy during scale-up. Anytime you move up in scale orders of magnitude, a process changes enough that it is considered a different process altogether.”&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;Westcott, continues, &lt;em&gt;“During the experiment, from the first sample that we took the analysis showed over a 99% conversion rate. There was some concern because I thought that that maybe was a little bit too high. So, we decided to take a second sample to see if it maintained a similar conversion rate. And we were a little surprised to find out that we actually did achieve this 99% conversion in our first 100-fold scale up of their process. That was very much an aha moment for us!&lt;/em&gt;”&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/interview-cayman-chemical-validates"&gt;&lt;img src="http://exozymes.twentythree.com/64968575/122969142/d406da6a433f2b61ac7a70d1464afefe/standard/download-39-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</media:description>
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            <title>Chief Scientific Officer, Tyler Korman, PhD</title>
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            <pubDate>Fri, 14 Nov 2025 00:11:36 GMT</pubDate>
            <media:title>Chief Scientific Officer, Tyler Korman, PhD</media:title>
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            <itunes:duration>03:42</itunes:duration>
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            <title>How eXoZymes uses AI - Tyler Korman (part 1)</title>
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            <pubDate>Tue, 11 Nov 2025 19:17:13 GMT</pubDate>
            <media:title>How eXoZymes uses AI - Tyler Korman (part 1)</media:title>
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            <itunes:author>eXoZymes</itunes:author>
            <itunes:duration>05:16</itunes:duration>
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            <title>eXoZymes launches subsidiary, NCTx, to unlock a promising compound for gut...</title>
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            <description>&lt;p&gt;&lt;p&gt;At the yearly SynBioBeta conference, eXoZymes Inc. (NASDAQ: EXOZ) (“eXoZymes”) - a pioneer of AI-engineered enzymes that can transform sustainable feedstock into nutraceuticals, medicines, and other essential chemicals - announced the formation of &lt;em&gt;‘NCTx, LLC’&lt;/em&gt; (NCTx), a purpose-built subsidiary company focused on the development and production of N-trans-caffeoyltyramine - a very rare, plant-derived compound with emerging relevance in the areas of metabolic health, gut integrity, and liver function.&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;Full story here:&amp;nbsp;&lt;a target="_blank" rel="noopener noreferrer nofollow" href="https://exozymes.com/blog/nctx-launch"&gt;https://exozymes.com/blog/nctx-launch&lt;/a&gt;&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/exozymes-launches-subsidiary-nctx"&gt;&lt;img src="http://exozymes.twentythree.com/64968580/112378102/1bd1b7fcaf04c5f612c07d24da9270db/standard/download-21-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</description>
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            <pubDate>Tue, 06 May 2025 22:18:50 GMT</pubDate>
            <media:title>eXoZymes launches subsidiary, NCTx, to unlock a promising compound for gut...</media:title>
            <itunes:summary>At the yearly SynBioBeta conference, eXoZymes Inc. (NASDAQ: EXOZ) (“eXoZymes”) - a pioneer of AI-engineered enzymes that can transform sustainable feedstock into nutraceuticals, medicines, and other essential chemicals - announced the formation of ‘NCTx, LLC’ (NCTx), a purpose-built subsidiary company focused on the development and production of N-trans-caffeoyltyramine - a very rare, plant-derived compound with emerging relevance in the areas of metabolic health, gut integrity, and liver function.Full story here:https://exozymes.com/blog/nctx-launch</itunes:summary>
            <itunes:subtitle>At the yearly SynBioBeta conference, eXoZymes Inc. (NASDAQ: EXOZ) (“eXoZymes”) - a pioneer of AI-engineered enzymes that can transform sustainable feedstock into nutraceuticals, medicines, and other essential chemicals - announced the formation of...</itunes:subtitle>
            <itunes:author>eXoZymes</itunes:author>
            <itunes:duration>03:19</itunes:duration>
            <media:description type="html">&lt;p&gt;&lt;p&gt;At the yearly SynBioBeta conference, eXoZymes Inc. (NASDAQ: EXOZ) (“eXoZymes”) - a pioneer of AI-engineered enzymes that can transform sustainable feedstock into nutraceuticals, medicines, and other essential chemicals - announced the formation of &lt;em&gt;‘NCTx, LLC’&lt;/em&gt; (NCTx), a purpose-built subsidiary company focused on the development and production of N-trans-caffeoyltyramine - a very rare, plant-derived compound with emerging relevance in the areas of metabolic health, gut integrity, and liver function.&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;Full story here:&amp;nbsp;&lt;a target="_blank" rel="noopener noreferrer nofollow" href="https://exozymes.com/blog/nctx-launch"&gt;https://exozymes.com/blog/nctx-launch&lt;/a&gt;&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/exozymes-launches-subsidiary-nctx"&gt;&lt;img src="http://exozymes.twentythree.com/64968580/112378102/1bd1b7fcaf04c5f612c07d24da9270db/standard/download-21-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</media:description>
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            <title>AI transforming biotechnology: From form with AlphaFold to function with...</title>
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            <description>&lt;p&gt;&lt;p&gt;Co-founder and VP of Development at eXoZymes, Dr. Paul Opgenorth, states:&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;"The Large Language Models (LLMs) actually got to learn our vocabulary by breaking down the English language to its core constituents - the 26 letters of the alphabet. Then the LLM identifies patterns and stitches the letters together in order to coherently answer the questions we give it. Conversely, you can think of enzymes in the same way. Enzymes are made up of 20 amino acids and LLMs can make the same correlations between these amino acids to get to stable structures. We can use these stable structures to make better enzymes and even select for properties that would up-classify them to exozymes."&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;Full story:&lt;/p&gt;&lt;p&gt;&lt;a target="_blank" rel="noopener noreferrer nofollow" href="https://exozymes.com/blog/alphafold-to-exozymes"&gt;https://exozymes.com/blog/alphafold-to-exozymes&lt;/a&gt;&lt;/p&gt;&lt;p&gt;#enzymes #exozymes #biomade #alphafold #proteinstructure&amp;nbsp;&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/ai-transforming-biotechnology-from"&gt;&lt;img src="http://exozymes.twentythree.com/64968568/112222080/907d40ec2fa6184b4e8499629bfb2e3e/standard/download-21-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</description>
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            <pubDate>Mon, 28 Apr 2025 20:47:39 GMT</pubDate>
            <media:title>AI transforming biotechnology: From form with AlphaFold to function with...</media:title>
            <itunes:summary>Co-founder and VP of Development at eXoZymes, Dr. Paul Opgenorth, states:"The Large Language Models (LLMs) actually got to learn our vocabulary by breaking down the English language to its core constituents - the 26 letters of the alphabet. Then the LLM identifies patterns and stitches the letters together in order to coherently answer the questions we give it. Conversely, you can think of enzymes in the same way. Enzymes are made up of 20 amino acids and LLMs can make the same correlations between these amino acids to get to stable structures. We can use these stable structures to make better enzymes and even select for properties that would up-classify them to exozymes."Full story:https://exozymes.com/blog/alphafold-to-exozymes#enzymes #exozymes #biomade #alphafold #proteinstructure</itunes:summary>
            <itunes:subtitle>Co-founder and VP of Development at eXoZymes, Dr. Paul Opgenorth, states:"The Large Language Models (LLMs) actually got to learn our vocabulary by breaking down the English language to its core constituents - the 26 letters of the alphabet. Then...</itunes:subtitle>
            <itunes:author>eXoZymes</itunes:author>
            <itunes:duration>04:04</itunes:duration>
            <media:description type="html">&lt;p&gt;&lt;p&gt;Co-founder and VP of Development at eXoZymes, Dr. Paul Opgenorth, states:&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;"The Large Language Models (LLMs) actually got to learn our vocabulary by breaking down the English language to its core constituents - the 26 letters of the alphabet. Then the LLM identifies patterns and stitches the letters together in order to coherently answer the questions we give it. Conversely, you can think of enzymes in the same way. Enzymes are made up of 20 amino acids and LLMs can make the same correlations between these amino acids to get to stable structures. We can use these stable structures to make better enzymes and even select for properties that would up-classify them to exozymes."&lt;/p&gt;&lt;p&gt;&lt;/p&gt;&lt;p&gt;Full story:&lt;/p&gt;&lt;p&gt;&lt;a target="_blank" rel="noopener noreferrer nofollow" href="https://exozymes.com/blog/alphafold-to-exozymes"&gt;https://exozymes.com/blog/alphafold-to-exozymes&lt;/a&gt;&lt;/p&gt;&lt;p&gt;#enzymes #exozymes #biomade #alphafold #proteinstructure&amp;nbsp;&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/ai-transforming-biotechnology-from"&gt;&lt;img src="http://exozymes.twentythree.com/64968568/112222080/907d40ec2fa6184b4e8499629bfb2e3e/standard/download-21-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</media:description>
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            <title>GEN Biotechnology: exozymes as the cover story in April 2025 issue</title>
            <link>http://exozymes.twentythree.com/gen-biotechnology-exozymes-as-the</link>
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            <pubDate>Mon, 21 Apr 2025 20:54:59 GMT</pubDate>
            <media:title>GEN Biotechnology: exozymes as the cover story in April 2025 issue</media:title>
            <itunes:summary></itunes:summary>
            <itunes:subtitle></itunes:subtitle>
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https://exozymes.com/blog/potential-impact#exozymes $EXOZ
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            <pubDate>Wed, 22 May 2024 16:33:18 GMT</pubDate>
            <media:title>The story of eXoZymes: Interview with Tyler Korman and Paul Opgenorth</media:title>
            <itunes:summary>Interviews with eXoZymes co-founders, Tyler Korman, PhD - and - Paul Opgenorth, PhD, sharing how the basic idea - liberating the enzymes from the confines of working in cells - was hatched in Jim Bowie's lab at UCLA, before being spun out as Invizyne Technologies in 2019 (rebranded to eXoZymes in 2025), including some of the projects and challenges along the way.</itunes:summary>
            <itunes:subtitle>Interviews with eXoZymes co-founders, Tyler Korman, PhD - and - Paul Opgenorth, PhD, sharing how the basic idea - liberating the enzymes from the confines of working in cells - was hatched in Jim Bowie's lab at UCLA, before being spun out as...</itunes:subtitle>
            <itunes:author>eXoZymes</itunes:author>
            <itunes:duration>06:56</itunes:duration>
            <media:description type="html">&lt;p&gt;&lt;p&gt;Interviews with eXoZymes co-founders, Tyler Korman, PhD - and - Paul Opgenorth, PhD, sharing how the basic idea - liberating the enzymes from the confines of working in cells - was hatched in Jim Bowie's lab at UCLA, before being spun out as Invizyne Technologies in 2019 (rebranded to eXoZymes in 2025), including some of the projects and challenges along the way.&lt;/p&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="http://exozymes.twentythree.com/the-story-of-exozymes-interview"&gt;&lt;img src="http://exozymes.twentythree.com/64968561/100951904/b84ec0d789f34490d6fac2a00e33164c/standard/download-45-thumbnail.jpg" width="75" height=""/&gt;&lt;/a&gt;&lt;/p&gt;</media:description>
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            <category>cannabinoids</category>
            <category>founder</category>
            <category>interview</category>
            <category>invizyne</category>
            <category>isobutanol</category>
            <category>jim bowie</category>
            <category>paul opgenorth</category>
            <category>SAF</category>
            <category>sustainable aviation fuel</category>
            <category>tyler korman</category>
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